Journal: Neuron

Article Title: Positional Strategies for Connection Specificity and Synaptic Organization in Spinal Sensory-Motor Circuits

doi: 10.1016/j.neuron.2019.04.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea pig polyclonal anti-vGluT1 (CU1705) This study RRID: AB_2750940 Guinea pig polyclonal anti-vGluT1 (CU1706) This study RRID: AB_2665455 Guinea pig polyclonal anti-vGluT1 (CU1707) This study RRID: AB_2750941 Rabbit polyclonal anti-dsRed Clontech Cat#632496 Goat polyclonal anti-Choline Acetyltransferase Millipore Cat#AB144P Chicken polyclonal anti-GFP Abcam Cat#AB13970 Rabbit polyclonal anti-GFP Invitrogen Cat#A11122 Sheep polyclonal anti-GFP AbD Serotec Cat#4745–1051 Goat polyclonal anti-Cholera Toxin, B-Subunit List Biologicals RRID: AB_10013220; Cat#703 Mouse monoclonal anti-Cholera Toxin, B-Subunit Abcam Cat#AB35988 Bacterial and Virus Strains scAAV6-CBh-GFP UNC Vector Core (VC) scAAV6-CBh-GFP scAAV6-CMV-Cre This study This study/ Custom UNC VC packaging scAAVI -CMV-Cre This study This study/ Custom UNC VC packaging scAAV9-CMV-Cre This study This study/ Custom UNC VC packaging Rabies SAD-B19 ΔG-GFP Wickersham et al., 2007 N/A Chemical, Peptides, and Recombinant Proteins Cholera Toxin B Subunit (low salt) List Biologicals Cat#104 Dextran, Fluorescein, 3000MW, Anionic, Lysine Fixable Invitrogen Cat#D-3306 Dextran, Tetramethylrhodamine, 3000MW, Anionic, Lysine Fixable Invitrogen Cat#D-3308 Experimental Models: Organisms/Strains Olig2::cre Dessaud et al., 2007 MGI:3774124 FoxP1 conditional Feng etal., 2010 N/A PV::FlpO Madisen et al., 2015 Jax 022730 Ai65 (RCFL-tdT) Madisen et al., 2015 Jax 021875 Ai14 (RCL-tdT) Madisen et al., 2010 Jax 007914 ChAT::cre Rossi et al., 2011 Jax 006410 Experimental Models: Cell Lines HEK293 Sigma Cat#85120602–1VL B7GG Zampieri et al., 2014 Ed Callaway Oligonucleotides FoxP1 Primer 1 5′ CTCCTAGTCACCTTCCCCAGTGC 3′ Feng et al., 2010 N/A FoxP1 Primer 2 5′ G AAC ACT GT CG AAT G ACCCTGC 3′ Feng et al., 2010 N/A Cre-F 5′ TTGCCGCGCCATCTGCCACCA 3′ This study N/A Cre-R 5′ CTAAT CG CC AT CTT CC AG 3′ This study N/A PVFlpO 16211 5′ TGTTTCTCCAGCATTTCCAG 3′ Madisen et al., 2015 N/A PVFlpO 17564 5′ GGATGCTTGCCGAAGATAAG 3′ Madisen et al., 2015 N/A PvFlpO 17566 5′ CTGAGCAGCTACATCAACAGG 3′ Madisen et al., 2015 N/A RosaTomWT-F 5′ AAGGGAGCTGCAGTGGAGTA 3′ Madisen et al., 2010 N/A RosaTomWT-R 5′ CCGAAAATCTGTGGGAAGTC 3′ Madisen et al., 2010 N/A RosaTomMut-F 5′ GGCATTAAAGCAGCGTATCC 3′ Madisen et al., 2010 N/A RosaTomMut-R 5′ CTGTTCCTGTACGGCATGG 3′ Madisen et al., 2010 N/A Software and Algorithms Imaris Bitplane N/A Open in a separate window KEY RESOURCES TABLE Sensory axonal and motor neuron dendritic morphologies show positional organization Axo-dendritic overlap reduces the number of potential post- synaptic partners The angle of axo-dendritic approach correlates with density of synapses A positional model predicts sensory-motor connectivity patterns with high accuracy

Techniques: Virus, Plasmid Preparation, Recombinant, Software

Journal: Neuron

Article Title: Positional Strategies for Connection Specificity and Synaptic Organization in Spinal Sensory-Motor Circuits

doi: 10.1016/j.neuron.2019.04.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea pig polyclonal anti-vGluT1 (CU1705) This study RRID: AB_2750940 Guinea pig polyclonal anti-vGluT1 (CU1706) This study RRID: AB_2665455 Guinea pig polyclonal anti-vGluT1 (CU1707) This study RRID: AB_2750941 Rabbit polyclonal anti-dsRed Clontech Cat#632496 Goat polyclonal anti-Choline Acetyltransferase Millipore Cat#AB144P Chicken polyclonal anti-GFP Abcam Cat#AB13970 Rabbit polyclonal anti-GFP Invitrogen Cat#A11122 Sheep polyclonal anti-GFP AbD Serotec Cat#4745–1051 Goat polyclonal anti-Cholera Toxin, B-Subunit List Biologicals RRID: AB_10013220; Cat#703 Mouse monoclonal anti-Cholera Toxin, B-Subunit Abcam Cat#AB35988 Bacterial and Virus Strains scAAV6-CBh-GFP UNC Vector Core (VC) scAAV6-CBh-GFP scAAV6-CMV-Cre This study This study/ Custom UNC VC packaging scAAVI -CMV-Cre This study This study/ Custom UNC VC packaging scAAV9-CMV-Cre This study This study/ Custom UNC VC packaging Rabies SAD-B19 ΔG-GFP Wickersham et al., 2007 N/A Chemical, Peptides, and Recombinant Proteins Cholera Toxin B Subunit (low salt) List Biologicals Cat#104 Dextran, Fluorescein, 3000MW, Anionic, Lysine Fixable Invitrogen Cat#D-3306 Dextran, Tetramethylrhodamine, 3000MW, Anionic, Lysine Fixable Invitrogen Cat#D-3308 Experimental Models: Organisms/Strains Olig2::cre Dessaud et al., 2007 MGI:3774124 FoxP1 conditional Feng etal., 2010 N/A PV::FlpO Madisen et al., 2015 Jax 022730 Ai65 (RCFL-tdT) Madisen et al., 2015 Jax 021875 Ai14 (RCL-tdT) Madisen et al., 2010 Jax 007914 ChAT::cre Rossi et al., 2011 Jax 006410 Experimental Models: Cell Lines HEK293 Sigma Cat#85120602–1VL B7GG Zampieri et al., 2014 Ed Callaway Oligonucleotides FoxP1 Primer 1 5′ CTCCTAGTCACCTTCCCCAGTGC 3′ Feng et al., 2010 N/A FoxP1 Primer 2 5′ G AAC ACT GT CG AAT G ACCCTGC 3′ Feng et al., 2010 N/A Cre-F 5′ TTGCCGCGCCATCTGCCACCA 3′ This study N/A Cre-R 5′ CTAAT CG CC AT CTT CC AG 3′ This study N/A PVFlpO 16211 5′ TGTTTCTCCAGCATTTCCAG 3′ Madisen et al., 2015 N/A PVFlpO 17564 5′ GGATGCTTGCCGAAGATAAG 3′ Madisen et al., 2015 N/A PvFlpO 17566 5′ CTGAGCAGCTACATCAACAGG 3′ Madisen et al., 2015 N/A RosaTomWT-F 5′ AAGGGAGCTGCAGTGGAGTA 3′ Madisen et al., 2010 N/A RosaTomWT-R 5′ CCGAAAATCTGTGGGAAGTC 3′ Madisen et al., 2010 N/A RosaTomMut-F 5′ GGCATTAAAGCAGCGTATCC 3′ Madisen et al., 2010 N/A RosaTomMut-R 5′ CTGTTCCTGTACGGCATGG 3′ Madisen et al., 2010 N/A Software and Algorithms Imaris Bitplane N/A Open in a separate window KEY RESOURCES TABLE Sensory axonal and motor neuron dendritic morphologies show positional organization Axo-dendritic overlap reduces the number of potential post- synaptic partners The angle of axo-dendritic approach correlates with density of synapses A positional model predicts sensory-motor connectivity patterns with high accuracy

Techniques: Plasmid Preparation, Recombinant, Software